Cyclic AMP relay and cyclic AMP-induced cyclic GMP accumulation during development ofDictyostelium discoideum

Cyclic Amp and Folic Acid Mediated Cyclic Gmp Accumulation In

In a suitable environment spores of the Dictyostelia [ 1 ] germinate yielding small amoebae. Each amoebae feeds on bacteria and divides. Following consumption of the food supply amoebae aggregate forming a slug where cells differentiate into spores and stalk ceils. The orientation of cells towards a food source or an aggregation center is guided by gradients of concentration of chemotactic mole...

Cyclic AMP and folic acid mediated cyclic GMP accumulation in Dictyostelium discoideum.

3':5'-cyclic AMP-dependent 3':5'-cyclic GMP accumulation in Dictyostelium discoideum.

Suspensions of 3':5'-cyclic AMP (cAMP)-sensitive cells of Dictyostelium discoideum responded to a cAMP pulse with increased 3':5'-cyclic GMP (cGMP) levels. Under the assay conditions used (2 x 10(8) cells per ml in 10 mM phosphate buffer, pH 6.0) cAMP (5 x 10(-8) M final concentration) increased cGMP levels from 1 pmol per 10(7) cells to 7 pmol per 10(7) cells in 10 sec and basal levels were re...

آدنوزین منوفسفات حلقوی ( Cyclic AMP )

Identification and cyclic AMP-induced modification of the cyclic AMP receptor in Dictyostelium discoideum.

We have recently identified a cell surface cAMP-binding protein by specific photoaffinity labeling of intact Dictyostelium discoideum cells with 8-N3-[32P] cAMP. The major photolabeled protein appears as a doublet (Mr = 40,000-43,000) in sodium dodecyl sulfate-polyacrylamide gel electrophoresis autoradiography. In this study, the doublet is shown to have the characteristics of the cAMP receptor...